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Gene Targeting and Transgenic Facility

The Gene Targeting and Transgenic Facility (GTTF) at UConn Health provides design and generation of genetically modified mice and other services.

GTTF mice on the cover!                                     New in the GTTF:

Cover of JBC

Canalis E, Schilling L, Yee SP, Lee SK, Zanotti S., (2016). Hajdu Cheney Mouse Mutants Exhibit Osteopenia, Increased Osteoclastogenesis and Bone Resorption. J. Biol. Chem. 291: 1538-1551.

Mice harboring a gain-of-function Notch2 mutation recapitulate selected skeletal manifestations of Hajdu-Cheney syndrome. Image of a femoral cross-section (bone marrow cells in green, bone surface with osteoblasts in purple and red, osteoclasts in yellow) from a mutant mouse.

CRISPR

Genetic Modification of mice using CRISPR-Cas9 technology is available as a GTTF service, and over 10 CRISPR mouse lines have already been successfully produced. Three of these projects involved insertion of large DNA fragments into targeted gene locations.  (Image adapted from: Corbyn, Z., Research: Biology's big hit. Nature (2015) 528: 54-55.)