Cre-loxP System
Reporter mice for Cre-LoxP system
Z/EG Double Reporter System
Strain Description: These transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being liver and lung tissue. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with enhanced GFP expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells.
Reference:
Novak A, Guo C, Yang W, Nagy A, Lobe CG.(2000). Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision.Genesis 2000 Nov-Dec;28(3-4):147-55.Jax stock number: 003920
Z/AP Double Reporter System
Strain Description: These transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being erythrocytes, chondrocytes and adipocytes. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with alkaline phosphatase expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity at the individual cell level.
Control Information
Reference:
1. Lobe CG, Koop KE, Kreppner W, Lomeli H, Gertsenstein M, Nagy A.(1999)Z/AP, a double reporter for cre-mediated recombination. Dev Biol 1999 Apr 15;208(2):281-92.
2 . Guo C, Yang W, Lobe CG.(2002). A Cre recombinase transgene with mosaic, widespread tamoxifen-inducible action.Genesis 2002 Jan;32(1):8-18Jax stock number: 003919