Mouse Embryonic Stem Cells
 

 

Services & Fees

Mouse Colony

Information

Protocols

Links

Contact Us

Home

 

  

 

R1 ES cells:

Established by Nagy in 1991 from (129/Svj x 129/Sv-CP) F1 male blastocyst. The GTTF currently has P12 available for electroporation.

Coat color of R1 ES cell generated mice:

129Sv-CP is a common name used for the 129S1/Sv-p<+> Tyr<+> Kitl<Sl-J>/+ strain. This strain is wild type at the p (pink-eyed dilution) and Tyr (tyrosinase) genes, and segregates for the Sl-J (steel Jackson) coat color mutation at the Kitl gene.

Mice from 129X1/SvJ strain, are either albino or pink-eyed, light-bellied, light chinchilla in color. This strain carries the A<w> (white bellied agouti allele homozygously), is homozygous for p (pink-eyed dilution), and is segregating for the c (albino) and c-ch (chinchilla) alleles of the Tyrosinase locus.

R1 ES cells did not receive the Kitl<Sl-J> mutation, but are wild type at the Kitl locus. The coat color expected from this ES cell contribution is white-bellied agouti. (Laura Trepanier, Jackson Lab)
     
 

TC-1 ES cells:

Derived from 129SVEVTac blastocyst by Phil Leder. We tested P16 and P22 TC-1 ES cells aggregation with CD-1 embryos. ES cells of both passage showed a strong contribution to chimeras and produced germline transmission chimeras. The GTTF currently has P12 TC-1 ES cells available for electroporation.
 

References:

Simpson EM, et al., 1997. Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nature Genetics 16:19-27.

Nagy, A., E. Gocza, E. Merentes-Diaz, V.R. Prideaux, E. Ivanyi, M. Markkula and J. Rossant (1990). Embryonic stem cells alone are able to support fetal development in the mouse. Development 110, 815-821.

Nomenclature for strain 129 mice

Threadgill DW, Yee D, Matin A, Nadeau JH and Magnuson (1997). Genealogy of the 129 inbred strains: 129SvJ is a contaminated inbred strain. Mammalian Genome 8,390-393. (Full Text )


Gene Targeting : A Practical Approach Joyner  AL, ed. 2000. IRL Press at Oxford University Press. New York. Oxford University Press; ISBN: 019963792X.