Mouse Embryonic Stem Cells Established at the GTTF
Mouse ES lines C:
Established in the GTTF from 129S6 (129SvEvTac) male blastocysts. C1 and C2 have been tested for germline transmission at P27.
Mouse ES lines D:
Established in the GTTF from 129S6 (129SvEvTac)/C57BL/6J F1 blastocysts. D1 and D2 have been tested for germline transmission at P27/28.
PrmCre ES lines:
Established in the GTTF from PrmCre (Jax Stock number: 003328) mouse line.
Status: Testing
Strain Description
Embryonic stem cells containing recombinase transgenes that are expressed in the male germ line, but not in other tissues or in the embryonic stem cells themselves, substantially simplify the production of subtle or conditional mutations in mice. This strain shows that transgenes comprised of the mouse protamine 1 promoter and the Cre recombinase coding sequence mediate the efficient recombination of a Cre target transgene in the male germ line, but not in other tissues. This system can be used for reducing the time, effort, and resources required to produce homologously recombined alleles in mice that have been secondarily rearranged by site-specific recombinase (JAX mouse database).