Site Specific Recombinase
FLPe
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This plasmid is carrying an enhanced Flp-Recombinase with improved activity driven via a CAGGS promoter. This promoter consists of a beta-actin promoter with the CMV IV enhancer upstream. Included downstream of Flpe are the EMCV IRES fused to the puromycin resistance gene, followed by the SV40 polyadenylation signal. The listed elements are lying 5´of the Flpe coding region: a splice acceptor site, a Kozak sequence and a nuclear localization signal from SV40 large-T antigen. This plasmid is appropriate for efficient excision of DNA stretches flanked by FRT sites e.g. for excision of a resistance cassette in a conditional construct/allele. The plasmid is carrying and Ampicillin resistance cassette. |
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Reference: schaft J, Aser-Padan R, van der Hoeven F, Gruss P and Stewart AF (2001) Efficient FLP Recombination in Mouse ES Cells and Oocytes. Genesis 31:6-10. (Full text) |
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