Gene Targeting & Transgenic Facility at UCHC

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ES Cells <-> embryo Aggregation


Please submit a completed Aggregation Request Form.

A fee for one ES cell line aggregation includes pre-aggregation ES cell culture, aggregation and housing chimeric pups until 3 wks old.

200 embryos will be aggregated for one line.

Investigators can also choose to do 2-3 ES cell lines in one aggregation day using 60-100 embryos for each line.

For the ES cell clones generated in the GTTF, we routinely do 2-3 lines in one aggregation. We guarantee germline transmission.

For ES cell lines grown at other facilities or labs, pathogen testing is required and should be paid by user. We connot garantee germline transmission.

Timeline for aggregation