ES
Cells <-> embryo Aggregation
Please submit a completed Aggregation Request Form.
A fee
for one ES cell line aggregation includes pre-aggregation ES cell
culture, aggregation and housing chimeric pups until 3 wks old.
200 embryos will
be aggregated for one line.
Investigators
can also choose to do 2-3 ES cell lines in one aggregation day using
60-100 embryos for each line.
For the ES cell
clones generated in the GTTF, we routinely do 2-3 lines in one aggregation.
We guarantee germline transmission.
For ES cell lines
grown at other facilities or labs, pathogen testing is required and
should be paid by user. We connot garantee germline transmission.
Timeline
for aggregation