Gene Targeting & Transgenic Facility
Services
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Gene Targeting & Transgenic Facility Services
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DNA
pronuclear microinjection Investigators who have transgene constructs ready should complete a DNA pronuclear microinjection request form. The microinjection will be scheduled after we have received the construct DNA and the completed request form. The full service includes DNA purification, microinjection and housing potential transgenic pups until 3 wks old. We guarantee 3 transgenic mice or 50 pups whichever comes first. Investigators can choose to use the service counted by injection days. There will be no guarantee for transgenic mice. At least 60 ug of circular construct DNA is needed. Please use Endofree plasmid kit, clontech nucleobond midiprep or CsCl-Ethidium Bromide gradients method to prepare your plasmid DNA. For BAC DNA please follow BAC preparation protocol. You can ship your DNA sample by FedEx at ambient temperature by adding 1/10 volume of NaAc 3M pH5.2 and 2 volume of EtOH 100% into your sample. Please seal the 1.5 ml tube well, put it into a 15 or 50 ml tube and then put it in an air bubble envelop. A small tube could be crushed easily during the shipment. The Fee schedule for DNA pronuclear microinjection can be found here. |
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