Gene Targeting & Transgenic Facility at UCHC
Services
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Gene Targeting & Transgenic Facility at UCHC Services |
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Rederivation of contaminated mouse lines
If the mouse line is maintained as heterozygous, 2-4 stud males between 6 wks to 6 months old are needed. The GTTF will provide superovulated embryo donors. The users should pick these donors at the GTTF and mate them with the stud males the day before rederivation. The GTTF will collect embryos and transfer the embryos to pseudopregnant recipients. If the mouse line is maintained as homozygous, 2-4 stud males between 6 wks to 6 months old and 4-6 females of 4wks old are needed. The female egg donors will be superovulated and mated with the stud males by the users. The GTTF will provide hormones and detail schedule and instruction for superovulation. The GTTF will collect embryos and transfer the embryos to pseudopregnant recipients. The fee for rederivation is here. The fee includes purchasing and housing donor and recipient female mice, housing vas males, superovulation of donors, embryo collection, embryo transfer, housing pups until 3 wks old and tail biopsy . Timeline for rederivation: |
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