Gene Targeting & Transgenic Facility at University of Connecticut Health Center
Gene Targeting & Transgenic Facility
at University of Connecticut Health Center
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Services & Fees
Information
Protocols
Mouse Colony
Links
Contact Us
Services
1. Design and Generation of targeting constructs: knock-out, knock-in, cko 2. ES cell targeting 3. ES cell <-> embryo aggregation 4. DNA pronuclear microinjection 5. DNA Pronuclear microinjection to mutant mouse embryos 6. Rederivation of contaminated mouse lines by embryo transfer 7. Tetraploid <-> ES cell aggregation 8. Mouse embryo cryopreservation 9. Import mouse lines by embryo transfer 10. Derivation of ES cell lines from mutant mice 11. Mouse colony management 12. Consultation 13. laser assisted IVF 14. Spermcryo 15. Chimera germline testing 16. PCR genotyping 17. Donors & pseudos 18. ES cells & Feeder media
Fees:
1. FY08 - Gene Trageting Fee Schedule for Fiscal year 08 2. FY08-2 - Trangenic and Others Fee Schedule for Fiscal year 08 3. FY07 - Gene Trageting Fee Schedule for Fiscal year 07 4. FY07-2 - Trangenic and Others Fee Schedule for Fiscal year 07
Information:
1. Mouse ES cell lines established in the GTTF 2. R1 ES Cells, TC-1 ES Cells, 3. Gene targeting vectors 4. BAC resourses 5. Cre-LoxP 6. Conditional genome alteration: List of resources available at the UCHC , Reporter mouse lines, conditional KO/expression vectors, Gene targeting related publications 7. Inbred vs Outbred Strains 8. Genetic Stability of Inbred mice 9. Congenic mouse strain 10. Transgene copy numbers 11. List of resources available at the UCHC 12. Reference Books 13. Gene targeting related publications 14. Info for external users
1. ES-Cell DNA Extraction From 96-well Plates 2. PCR screening for targeted ES colonies in 96-well 3. Rating of 96-well Plate ES cell Genomic DNA Digestion Enzymes 4. Tail DNA Extraction 5. Ear punches/yolk sacs/tail DNA for PCR 6. DNA preparation for PCR products less than 500 bp 7. Genotyping for Z/AP and Z/EG mice 8. PCR conditions for genotypoing
Mouse Colony:
1. SOP for mouse colony management 2. SOP for colony management 3. Ear Notch 4. Know your mice 5. signs of illness 6. Husbandry info 7. SOP for GTTF colony husbandry 8. Database access
LAIVF, sperm Cryo Shipping ( mice/embryos - need to pay for cryo & shipping envelope) PCR genotyping
1. UCHC 2. Farmington 3. Team Members 4. Mouse Colony
Our Accomplishments
