Protocols
PCR Conditions for Genotyping
You will receive primer sequences for the gene targeting mice. The following conditions are used for PCR genotyping for all our gene targeting mice. We'll email you if special conditions are used for your mice.
PCR Mix:
Stocks
dNTP 10mM
10x PCR buffer
Primers 10 uM
Taq polymerase
H2O
Total volume
One reaction
0.25 µl
1.25 µl
0.3 µl each
0.06 µl
9.48 µl
12.02 µl
(make for 11 reactions)
2.75 µl
13.75 µl
3.3 µl each
0.66 µl
104.28 µl
124.74 µl
Add DNA 0.5 µl
PCR Cycles:
1. Denaturation:
2. Denaturation:
3. Annealing:
4. Extension:
2-4 steps X 35 cycles
5. Extension
94°C
94°C
55 °C
72 °C
72°C
3 min
30 sec
30 sec
30 sec
5 min