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Genotyping for Z/AP and Z/EG Transgenic Mice

  • Earpunch piece into PBS in a 96-well plate.
  • Fix for ten minutes in 0.2 percent glutaraldehyde in PBS at room temperature.
  • Wash three times with PBS - five minutes each time.
  • Add X-gal staining solution 100 ul.
  • Leave the plate at 37C or room temperature overnight protected from light.
X-gal Staining Solution
10 ml
25 ml
50 ml
Final Concentration
PBS 9.350 23.375 46.750  
X-gal (in DMSO, 40 mg/ml) 0.250 0.625 1.250 1 mg/ml
K3Fe(CN)6 (500 mM) 0.100 0.250 0.500 5 mM
K4Fe(CN)6 (500 mM) 0.100 0.250 0.500 5 mM
MgCl2 (100 mM) 0.200 0.500 1.000 2 mM